Sense and nonsense of high-temperature liquid chromatography.

نویسندگان

  • Sabine Heinisch
  • Jean-Louis Rocca
چکیده

High-temperature liquid chromatography (HTLC) is recognized today as a valuable technique in reversed-phase high-performance liquid chromatography (RP-HPLC). Column temperature can play a role in reducing analysis time, modifying retention, controlling selectivity, changing efficiency or improving detection sensitivity. The different effects of high temperatures on reversed-phase separations, the practical limitations due to the instrumentation, the limits and the main advantages of HTLC, especially for the separation of polar and ionized compounds, are reviewed.

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عنوان ژورنال:
  • Journal of chromatography. A

دوره 1216 4  شماره 

صفحات  -

تاریخ انتشار 2009